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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in several tumors, which ends up in chromosome instability, and thus may possibly lead to tumorigenesis. This protein was discovered to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important position in marketing mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been explained. Two extra splice variants were being documented but specific nucleotide sequence data isn't nonetheless offered.
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Blocking peptides are peptides that bind exclusively to the goal antibody and block antibody binding. These peptide commonly consists of the epitope identified via the antibody.
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This guideline outlines even further attainable optimization of cellular and immunoassay parameters to make sure the very best benefits are...
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This tutorial outlines further more possible optimization of mobile and immunoassay parameters to ensure the best possible outcomes are...
Antibodies bound to the blocking peptide no longer bind towards the epitope around the target protein. By evaluating the staining within the blocked antibody vs . the antibody by itself, you can see which staining is specific.
Goal Data Cyclin E belongs to your extremely conserved cyclin family members, whose members click here are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show unique expression and degradation patterns which add on the temporal coordination of each mitotic event. Cyclin E varieties a fancy with and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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